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Isolation and sequence-specific DNA binding of the Antennapedia homeodomain.

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dc.contributor.authorMüller, M.en
dc.contributor.authorAffolter, M.en
dc.contributor.authorLeupin, W.en
dc.contributor.authorOtting, G.en
dc.contributor.authorWüthrich, K.en
dc.contributor.authorGehring, W. J.en
dc.date.accessioned2026-03-01T17:42:20Z
dc.date.available2026-03-01T17:42:20Z
dc.date.issued1988-12-20en
dc.description.abstractThe homeodomain encoded by the Antennapedia (Antp) gene of Drosophila was overproduced in a T7 expression vector in Escherichia coli. The corresponding polypeptide of 68 amino acids was purified to homogeneity. The homeodomain was analysed by ultracentrifugation and assayed for DNA binding. The secondary structure of the isolated homeodomain was determined by nuclear magnetic resonance spectroscopy. DNA-binding studies indicate that the isolated homeodomain binds to DNA in vitro. It selectively binds to the same sites as a longer Antp polypeptide and a full-length fushi tarazu (ftz) protein. Therefore, the homeodomain represents the DNA-binding domain of the homeotic proteins.en
dc.description.statusPeer-revieweden
dc.format.extent6en
dc.identifier.issn0261-4189en
dc.identifier.otherPubMed:2907479en
dc.identifier.otherORCID:/0000-0002-0563-0146/work/206892066en
dc.identifier.scopus0342759569en
dc.identifier.urihttps://hdl.handle.net/1885/733806859
dc.language.isoenen
dc.sourceEMBO Journalen
dc.titleIsolation and sequence-specific DNA binding of the Antennapedia homeodomain.en
dc.typeJournal articleen
dspace.entity.typePublicationen
local.bibliographicCitation.lastpage4304en
local.bibliographicCitation.startpage4299en
local.contributor.affiliationMüller, M.; University of Baselen
local.contributor.affiliationAffolter, M.; University of Baselen
local.contributor.affiliationLeupin, W.; University of Baselen
local.contributor.affiliationOtting, G.; University of Baselen
local.contributor.affiliationWüthrich, K.; University of Baselen
local.contributor.affiliationGehring, W. J.; University of Baselen
local.identifier.citationvolume7en
local.identifier.doi10.1002/j.1460-2075.1988.tb03328.xen
local.identifier.pureb97b3d32-7ff0-41fb-a8f8-fb7ac94f35c3en
local.identifier.urlhttps://www.scopus.com/pages/publications/0342759569en
local.type.statusPublisheden

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