Kurdyukov, Sergey; Mathesius, Ulrike; Nolan, Kim E; Sheahan, Michael B; Goffard, Nicolas; Carroll, Bernard J; Rose, Ray J
BACKGROUND The Medicago truncatula 2HA seed line is highly embryogenic while the parental line Jemalong rarely produces embryos. The 2HA line was developed from one of the rare Jemalong regenerates and this method for obtaining a highly regenerable genotype in M. truncatula is readily reproducible suggesting an epigenetic mechanism. Microarray transcriptomic analysis showed down regulation of an ETHYLENE INSENSITIVE 3-like gene in 2HA callus which provided an approach to investigating...[Show more]
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