Simple Method for Determination of Thiocyanate in Urine
Haque, Memdadul; Bradbury, James
Description
Background: It would be useful to develop a simple kit method for determination of thiocyanate in urine, which could be used to monitor cyanide overload in cassava-consuming populations. Methods: The method was based on the quantitative oxidation of thiocyanate in acid permanganate at room temperature in a closed vial with liberation of HCN, which reacted with a picrate paper. For semiquantitative analysis in the field, the colored picrate paper was matched with a color chart prepared using...[Show more]
dc.contributor.author | Haque, Memdadul | |
---|---|---|
dc.contributor.author | Bradbury, James | |
dc.date.accessioned | 2015-12-13T23:22:33Z | |
dc.date.available | 2015-12-13T23:22:33Z | |
dc.identifier.issn | 0009-9147 | |
dc.identifier.uri | http://hdl.handle.net/1885/91507 | |
dc.description.abstract | Background: It would be useful to develop a simple kit method for determination of thiocyanate in urine, which could be used to monitor cyanide overload in cassava-consuming populations. Methods: The method was based on the quantitative oxidation of thiocyanate in acid permanganate at room temperature in a closed vial with liberation of HCN, which reacted with a picrate paper. For semiquantitative analysis in the field, the colored picrate paper was matched with a color chart prepared using known amounts of KSCN. In the laboratory, a more accurate result was obtained by elution of the colored complex in water and measurement of the absorbance at 510 nm. Over the range 0-100 mg/L, there was a linear relationship given by the equation: thiocyanate content (mg/L) = 78 x absorbance. Results: The picrate thiocyanate method gave no interference with urine samples containing protein at less than 7 g/L, 21 amino acids, histamine, glucose, NaCl, urea, blood, and linamarin. For 53 urine samples analyzed by an accurate column method and the thiocyanate picrate method, a regression line gave very good agreement (r2 = 1.000). Quantitative recoveries of thiocyanate added to urine samples were obtained with the picrate method. Conclusions: A simple picrate kit for determination of thiocyanate in urine was developed and is available free of charge for workers in developing countries. | |
dc.publisher | American Association of Clinical Chemistry | |
dc.source | Clinical Chemistry | |
dc.subject | Keywords: amino acid; cyanide; glucose; histamine; linamarin; picric acid; sodium chloride; thiocyanate; urea; accuracy; article; cassava; cyanide poisoning; developing country; female; food composition; food poisoning; human; major clinical study; male; urinalysis | |
dc.title | Simple Method for Determination of Thiocyanate in Urine | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.description.refereed | Yes | |
local.identifier.citationvolume | 45 | |
dc.date.issued | 1999 | |
local.identifier.absfor | 060101 - Analytical Biochemistry | |
local.identifier.ariespublication | MigratedxPub22267 | |
local.type.status | Published Version | |
local.contributor.affiliation | Haque, Memdadul, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Bradbury, James, College of Medicine, Biology and Environment, ANU | |
local.bibliographicCitation.startpage | 1459 | |
local.bibliographicCitation.lastpage | 1464 | |
dc.date.updated | 2015-12-12T09:11:32Z | |
local.identifier.scopusID | 2-s2.0-0032860534 | |
Collections | ANU Research Publications |
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