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Sensitivity-enhanced double-TROSY experiment for simultaneous measurement of one-bond 15 N- 1 H, 15 N- 13 C and two-bond 1 H- 13 C couplings

Hoshino, Masaru; Otting, Gottfried


A recently published experiment for the measurement of 1HN, 1NC', and 2JHC' coupling constants [J. Am. Chem. Soc. 125 (2003) 11504] was modified to yield a double-TROSY experiment which selects 1 of the 16 multiplet components from a 15N-HSQC spectrum recorded of a uniformly 15N/ 13C-labelled protein. Subspectra containing any 1 of the 16 multiplet components can be generated allowing accurate coupling constant measurements. The experiment is sensitivity enhanced, turning all magnetization...[Show more]

CollectionsANU Research Publications
Date published: 2004
Type: Journal article
Source: Journal of Magnetic Resonance
DOI: 10.1016/j.jmr.2004.08.020


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