An alternative strategy for the storage and transport of sputum specimens for Mycobacterium Tuberculosis drug resistance surveys
Lumb, Richard; Ardian, M; Waramori, G; Syahrial, H; Tjitra, Emiliana; Maguire, Graeme; Anstey, Nicholas; Kelly, Paul
Description
SETTING: A district level tuberculosis (TB) programme in Indonesia. OBJECTIVE: To evaluate whether a single sputum specimen could be stored by refrigeration for an extended period of time, then transported to a reference laboratory and successfully cultured for Mycobacterium tuberculosis. METHODS: Single sputum specimens were collected from newly diagnosed smear-positive pulmonary TB patients, refrigerated at the study site without addition of 1% cetylpyridinium chloride, batched and sent to...[Show more]
dc.contributor.author | Lumb, Richard | |
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dc.contributor.author | Ardian, M | |
dc.contributor.author | Waramori, G | |
dc.contributor.author | Syahrial, H | |
dc.contributor.author | Tjitra, Emiliana | |
dc.contributor.author | Maguire, Graeme | |
dc.contributor.author | Anstey, Nicholas | |
dc.contributor.author | Kelly, Paul | |
dc.date.accessioned | 2015-12-07T22:55:13Z | |
dc.identifier.issn | 1027-3719 | |
dc.identifier.uri | http://hdl.handle.net/1885/28297 | |
dc.description.abstract | SETTING: A district level tuberculosis (TB) programme in Indonesia. OBJECTIVE: To evaluate whether a single sputum specimen could be stored by refrigeration for an extended period of time, then transported to a reference laboratory and successfully cultured for Mycobacterium tuberculosis. METHODS: Single sputum specimens were collected from newly diagnosed smear-positive pulmonary TB patients, refrigerated at the study site without addition of 1% cetylpyridinium chloride, batched and sent to the reference laboratory, where they were decontaminated and inoculated into BACTEC MGIT 960 liquid media. RESULTS: One hundred and seven patients were enrolled. The median specimen storage time was 12 days (range 1-38) and median transportation time was 4 days (2-12). The median time from specimen collection until processing was 18 days (4-42). Only 4 (3.7%) specimens failed to grow Mycobacterium species and M. tuberculosis was isolated from 101 (94.4%) specimens. Six specimens with breakthrough contamination successfully grew M. tuberculosis after a second decontamination procedure. CONCLUSIONS: Single sputum specimens collected at a remote setting, refrigerated for relatively long periods without preservatives and transported without refrigeration to a reference laboratory can yield a high positive culture rate. These findings offer potential logistic simplification and cost savings for drug resistance surveys in low-resource countries. | |
dc.publisher | International Union against Tuberculosis and Lung Disease | |
dc.source | International Journal of Tuberculosis and Lung Disease | |
dc.subject | Keywords: cetylpyridinium salt; bacterium contamination; freezing; human; laboratory diagnosis; lung tuberculosis; major clinical study; Mycobacterium tuberculosis; priority journal; review; sputum culture; storage; time; Data Collection; Drug Resistance, Bacterial Drug resistance; Specimens; Sputum; Survey; Tuberculosis | |
dc.title | An alternative strategy for the storage and transport of sputum specimens for Mycobacterium Tuberculosis drug resistance surveys | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 10 | |
dc.date.issued | 2006 | |
local.identifier.absfor | 111706 - Epidemiology | |
local.identifier.ariespublication | u4054856xPUB57 | |
local.type.status | Published Version | |
local.contributor.affiliation | Lumb, Richard, Institute of Medical and Veterinary Science | |
local.contributor.affiliation | Ardian, M, District Ministry of Health | |
local.contributor.affiliation | Waramori, G, Public Health & Malaria Control Department | |
local.contributor.affiliation | Syahrial, H, National Institute of Health Research and Development | |
local.contributor.affiliation | Tjitra, Emiliana, Ministry of Health, Indonesia | |
local.contributor.affiliation | Maguire, Graeme, Menzies School of Health Research | |
local.contributor.affiliation | Anstey, Nicholas, Menzies School of Health Research | |
local.contributor.affiliation | Kelly, Paul, College of Medicine, Biology and Environment, ANU | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.issue | 2 | |
local.bibliographicCitation.startpage | 172 | |
local.bibliographicCitation.lastpage | 177 | |
dc.date.updated | 2015-12-07T12:53:42Z | |
local.identifier.scopusID | 2-s2.0-33244466310 | |
Collections | ANU Research Publications |
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