Optimising the linear electron transport rate measured by chlorophyll a fluorescence to empirically match the gross rate of oxygen evolution in white light: towards improved estimation of the cyclic electron flux around photosystem I in leaves
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zhang, Meng meng; Fan, Da Yong; Sun, Guang-Yu; Chow, Wah S (Fred)
Description
The cyclic electron flux (CEF) around photosystem I (PSI) was discovered in isolated chloroplasts more than six decades ago, but its quantification has been hampered by the absence of net formation of a product or net consumption of a substrate. We estimated in vivo CEF in leaves as the difference (ΔFlux) between the total electron flux through PSI (ETR1) measured by a near infrared signal, and the linear electron flux through both photosystems by optimised measurement of chlorophyll a...[Show more]
dc.contributor.author | zhang, Meng meng | |
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dc.contributor.author | Fan, Da Yong | |
dc.contributor.author | Sun, Guang-Yu | |
dc.contributor.author | Chow, Wah S (Fred) | |
dc.date.accessioned | 2019-07-29T05:41:09Z | |
dc.identifier.issn | 1445-4408 | |
dc.identifier.uri | http://hdl.handle.net/1885/164766 | |
dc.description.abstract | The cyclic electron flux (CEF) around photosystem I (PSI) was discovered in isolated chloroplasts more than six decades ago, but its quantification has been hampered by the absence of net formation of a product or net consumption of a substrate. We estimated in vivo CEF in leaves as the difference (ΔFlux) between the total electron flux through PSI (ETR1) measured by a near infrared signal, and the linear electron flux through both photosystems by optimised measurement of chlorophyll a fluorescence (LEFfl). Chlorophyll fluorescence was excited by modulated green light from a light-emitting diode at an optimal average irradiance, and the fluorescence was detected at wavelengths >710 nm. In this way, LEFfl matched the gross rate of oxygen evolution multiplied by 4 (LEFO2) in broad-spectrum white actinic irradiance up to half (spinach, poplar and rice) or one third (cotton) of full sunlight irradiance. This technique of estimating CEF can be applied to leaves attached to a plant. | |
dc.description.sponsorship | This work was supported by a China Scholarship Council Fellowship (to M-M. Z.), and a grant from the Australian Research Council (to WSC, DP1093827). | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en_AU | |
dc.publisher | CSIRO Publishing | |
dc.rights | © CSIRO 2018 | |
dc.source | Functional Plant Biology | |
dc.title | Optimising the linear electron transport rate measured by chlorophyll a fluorescence to empirically match the gross rate of oxygen evolution in white light: towards improved estimation of the cyclic electron flux around photosystem I in leaves | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 45 | |
dc.date.issued | 2018 | |
local.identifier.absfor | 060705 - Plant Physiology | |
local.identifier.ariespublication | u4485658xPUB2345 | |
local.publisher.url | http://www.publish.csiro.au/ | |
local.type.status | Accepted Version | |
local.contributor.affiliation | Zhang, Meng-Meng, College of Science, ANU | |
local.contributor.affiliation | Fan, Da Yong, College of Science, ANU | |
local.contributor.affiliation | Sun, Guang-Yu, Northeast Forestry University | |
local.contributor.affiliation | Chow, Wah S (Fred), College of Science, ANU | |
dc.relation | http://purl.org/au-research/grants/arc/DP1093827 | |
local.bibliographicCitation.issue | 11 | |
local.bibliographicCitation.startpage | 1138 | |
local.bibliographicCitation.lastpage | 1148 | |
local.identifier.doi | 10.1071/FP18039 | |
local.identifier.absseo | 829999 - Plant Production and Plant Primary Products not elsewhere classified | |
local.identifier.absseo | 970106 - Expanding Knowledge in the Biological Sciences | |
dc.date.updated | 2019-03-31T07:23:20Z | |
local.identifier.thomsonID | 000446654300006 | |
dcterms.accessRights | Open Access | |
dc.provenance | http://sherpa.ac.uk/romeo/issn/1445-4408/..."author can archive post-print (ie final draft post-refereeing)" from SHERPA/RoMEO site (as at 23/08/19). | |
Collections | ANU Research Publications |
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File | Description | Size | Format | Image |
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Zhang et al Revised submitted.pdf | 704.36 kB | Adobe PDF |
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