Techniques for labelling biological macromolecules for spectroscopic studies
The ability to express and purify soluble protein in significant amounts is a prerequisite for the structure analysis of biological macromolecules by spectroscopic techniques. Nuclear magnetic resonance (NMR) and electron paramagnetic resonance (EPR) spectroscopies are powerful biophysical techniques which are widely used in structural biology. This thesis focuses on the use of different fusion constructs and tagging strategies to produce samples for...[Show more]
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