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Improved two-photon imaging of living neurons in brain tissue through temporal gating

Gautam, Vini; Choy, Julian; Stricker, Christian; Bachor, Hans; Daria, Vincent; Drury, Jack Duncan


We optimize two-photon imaging of living neurons in brain tissue by temporally gating an incident laser to reduce the photon flux while optimizing the maximum fluorescence signal from the acquired images. Temporal gating produces a bunch of ~10 femtosecond pulses and the fluorescence signal is improved by increasing the bunch-pulse energy. Gating is achieved using an acousto-optic modulator with a variable gating frequency determined as integral multiples of the imaging sampling frequency. We...[Show more]

CollectionsANU Research Publications
Date published: 2015
Type: Journal article
Source: Biomedical Optics Express
DOI: 10.1364/BOE.6.004027


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