Analysis of the solution conformations of T4 lysozyme by paramagnetic NMR spectroscopy
A large number of crystal structures of bacteriophage T4 lysozyme (T4-L) have shown that it contains two subdomains, which can arrange in a compact conformation (closed state) or, in mutants of T4-L, more extended structures (open state). In solution, wild-type T4-L displays only a single set of nuclear magnetic resonance (NMR) signals, masking any conformational heterogeneity. To probe the conformational space of T4-L, we generated a site-specific lanthanide binding site by attaching...[Show more]
|Collections||ANU Research Publications|
|Source:||Physical chemistry chemical physics : PCCP|
|PhysChemChemPhys2016_18_5850.pdf||4.1 MB||Adobe PDF|
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