Truncation of gene F5L partially masks rescue of vaccinia virus strain MVA growth on mammalian cells by restricting plaque size
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Dobson, Bianca; Tscharke, David C.
Description
Modified vaccinia virus Ankara (MVA) is a candidate vaccine vector that is severely attenuated due to mutations acquired during several hundred rounds of serial passage in vitro. A previous study used marker rescue to produce a set of MVA recombinants with improved replication on mammalian cells. Here, we extended the characterization of these rescued MVA strains and identified vaccinia virus (VACV) gene F5L as a determinant of plaque morphology but not replication in vitro. F5 joins a growing...[Show more]
dc.contributor.author | Dobson, Bianca | |
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dc.contributor.author | Tscharke, David C. | |
dc.date.accessioned | 2014-02-24T04:44:09Z | |
dc.identifier.issn | 0022-1317 | |
dc.identifier.uri | http://hdl.handle.net/1885/11416 | |
dc.description.abstract | Modified vaccinia virus Ankara (MVA) is a candidate vaccine vector that is severely attenuated due to mutations acquired during several hundred rounds of serial passage in vitro. A previous study used marker rescue to produce a set of MVA recombinants with improved replication on mammalian cells. Here, we extended the characterization of these rescued MVA strains and identified vaccinia virus (VACV) gene F5L as a determinant of plaque morphology but not replication in vitro. F5 joins a growing group of VACV proteins that influence plaque formation more strongly than virus replication and which are disrupted in MVA. These defective genes in MVA confound the interpretation of marker rescue experiments designed to map mutations responsible for the attenuation of this important VACV strain. | |
dc.description.sponsorship | This work was funded by grants to DCT: NHMRC APP1023141 and ARC FT110100310. | |
dc.format | 16 pages | |
dc.publisher | Society for General Microbiology | |
dc.source | Journal of General Virology 95 (Pt 2) (2014):466-71 | |
dc.subject | vaccinia virus | |
dc.subject | modified vaccinia virus Ankara | |
dc.subject | MVA | |
dc.subject | plaque morphology | |
dc.title | Truncation of gene F5L partially masks rescue of vaccinia virus strain MVA growth on mammalian cells by restricting plaque size | |
dc.type | Journal article | |
local.identifier.citationvolume | 95 | |
dcterms.dateAccepted | 2013-10-20 | |
dc.date.issued | 2014-02 | |
local.identifier.absfor | 060506 - Virology | |
local.identifier.ariespublication | U3488905xPUB1840 | |
local.publisher.url | http://www.sgm.ac.uk/ | |
local.type.status | Accepted Version | |
local.contributor.affiliation | Dobson, Bianca M, Division of Biomedical Science and Biochemistry, Research School of Biology, The Australian National University | |
local.contributor.affiliation | Tscharke, David C., Division of Biomedical Science and Biochemistry, Research School of Biology, The Australian National University | |
dc.relation | http://purl.org/au-research/grants/nhmrc/1023141 | |
dc.relation | http://purl.org/au-research/grants/arc/FT110100310 | |
local.request.email | david.tscharke@anu.edu.au | |
local.request.name | Tscharke, David C. | |
local.bibliographicCitation.issue | PART 2 | |
local.bibliographicCitation.startpage | 466 | |
local.bibliographicCitation.lastpage | 471 | |
local.identifier.doi | 10.1099/vir.0.058495-0 | |
local.identifier.absseo | 920109 - Infectious Diseases | |
dc.date.updated | 2015-12-10T11:19:35Z | |
local.identifier.scopusID | 2-s2.0-84892617514 | |
local.identifier.thomsonID | 000338177400023 | |
dcterms.accessRights | Open Access | |
dc.provenance | http://www.sherpa.ac.uk/romeo/issn/0022-1317/... "subject to 12 month embargo, author can archive post-print (ie final draft post-refereeing)" From Sherpa/Romeo (as at 24/2/14). | |
Collections | ANU Research Publications |
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File | Description | Size | Format | Image |
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Dobson and Tscharke Truncation of gene 2014.pdf | Author/s Accepted Manuscript (AAM) | 1.4 MB | Adobe PDF |
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