Nitric oxide plays a critical role in the recovery of Lewis rats from experimental autoimmune encephalomyelitis and the maintenance of resistance to reinduction
O'Brien, Nikki; Charlton, Brett; Cowden, William; Willenborg, David
Description
Experimental autoimmune encephalomyelitis (EAE) is a T cell-mediated autoimmune disease of the CNS and an animal model for the human demyelinating disease, multiple sclerosis. In the Lewis rat, myelin basic protein (MBP)- CFA-induced EAE is an acute monophasic disease from which animals recover fully, do not relapse, and develop a robust long-term resistance to further active reinduction of disease. In this paper, we report that rats recovering from MBP-CFA-induced EAE have significantly...[Show more]
dc.contributor.author | O'Brien, Nikki | |
---|---|---|
dc.contributor.author | Charlton, Brett | |
dc.contributor.author | Cowden, William | |
dc.contributor.author | Willenborg, David | |
dc.date.accessioned | 2015-12-13T23:25:07Z | |
dc.identifier.issn | 0022-1767 | |
dc.identifier.uri | http://hdl.handle.net/1885/92524 | |
dc.description.abstract | Experimental autoimmune encephalomyelitis (EAE) is a T cell-mediated autoimmune disease of the CNS and an animal model for the human demyelinating disease, multiple sclerosis. In the Lewis rat, myelin basic protein (MBP)- CFA-induced EAE is an acute monophasic disease from which animals recover fully, do not relapse, and develop a robust long-term resistance to further active reinduction of disease. In this paper, we report that rats recovering from MBP-CFA-induced EAE have significantly increased serum levels of reactive nitrogen intermediates indicative of increased NO production. These levels remain elevated after the recovery period and increase even further early after a rechallenge with MBP-CFA, and all animals are totally refractory to a second episode of disease. Oral treatment of rats with N- methyl-L-arginine acetate (L-NMA), beginning at peak disease on day 11 postimmunization, results in significant prolongation of disease and an alteration in the presentation of clinical symptoms from that of solely hind limb paresis/paralysis to severe fore limb involvement as well. Treatment of fully recovered rats with L-NMA 24 h before a rechallenge with MBP-CFA leads to decreased serum reactive nitrogen intermediate levels and results in a second episode of EAE in 100% of animals. Furthermore, L-NMA treatment of fully recovered rats in the absence of a rechallenge immunization leads to spontaneous relapse of disease. | |
dc.publisher | American Association of Immunologists | |
dc.source | Journal of Immunology | |
dc.subject | Keywords: nitric oxide; allergic encephalomyelitis; animal experiment; animal model; animal tissue; article; autoimmune disease; controlled study; experimental model; female; immunization; multiple sclerosis; nonhuman; paresis; priority journal; rat; relapse; Admin | |
dc.title | Nitric oxide plays a critical role in the recovery of Lewis rats from experimental autoimmune encephalomyelitis and the maintenance of resistance to reinduction | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.description.refereed | Yes | |
local.identifier.citationvolume | 163 | |
dc.date.issued | 1999 | |
local.identifier.absfor | 110703 - Autoimmunity | |
local.identifier.ariespublication | MigratedxPub23642 | |
local.type.status | Published Version | |
local.contributor.affiliation | O'Brien, Nikki, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Charlton, Brett, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Cowden, William, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Willenborg, David, College of Medicine, Biology and Environment, ANU | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.issue | 12 | |
local.bibliographicCitation.startpage | 6841 | |
local.bibliographicCitation.lastpage | 7 | |
dc.date.updated | 2015-12-12T09:23:35Z | |
local.identifier.scopusID | 2-s2.0-0032715755 | |
Collections | ANU Research Publications |
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