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Carbon allocation to major metabolites in illuminated leaves is not just proportional to photosynthesis when gaseous conditions (CO2 and O2) vary

Abadie, Cyril; Bathellier, Camille; Tcherkez, Guillaume

Description

In gas-exchange experiments, manipulating CO2 and O2 is commonly used to change the balance between carboxylation and oxygenation. Downstream metabolism (utilization of photosynthetic and photorespiratory products) may also be affected by gaseous conditions but this is not well documented. Here, we took advantage of sunflower as a model species, which accumulates chlorogenate in addition to sugars and amino acids (glutamate, alanine, glycine and serine). We performed isotopic labelling with...[Show more]

dc.contributor.authorAbadie, Cyril
dc.contributor.authorBathellier, Camille
dc.contributor.authorTcherkez, Guillaume
dc.date.accessioned2021-05-13T00:24:56Z
dc.date.available2021-05-13T00:24:56Z
dc.identifier.issn0028-646X
dc.identifier.urihttp://hdl.handle.net/1885/232989
dc.description.abstractIn gas-exchange experiments, manipulating CO2 and O2 is commonly used to change the balance between carboxylation and oxygenation. Downstream metabolism (utilization of photosynthetic and photorespiratory products) may also be affected by gaseous conditions but this is not well documented. Here, we took advantage of sunflower as a model species, which accumulates chlorogenate in addition to sugars and amino acids (glutamate, alanine, glycine and serine). We performed isotopic labelling with 13CO2 under different CO2/O2 conditions, and determined 13C contents to compute 13C-allocation patterns and build-up rates. The 13C content in major metabolites was not found to be a constant proportion of net fixed carbon but, rather, changed dramatically with CO2 and O2. Alanine typically accumulated at low O2 (hypoxic response) while photorespiratory intermediates accumulated under ambient conditions and at high photorespiration, glycerate accumulation exceeding serine and glycine build-up. Chlorogenate synthesis was relatively more important under normal conditions and at high CO2 and its synthesis was driven by phosphoenolpyruvate de novo synthesis. These findings demonstrate that carbon allocation to metabolites other than photosynthetic end products is affected by gaseous conditions and therefore the photosynthetic yield of net nitrogen assimilation varies, being minimal at high CO2 and maximal at high O2.
dc.description.sponsorshipWe thank the Australian Research Council for its support via a Future Fellowship awarded to G.T. under contract FT140100645.
dc.format.mimetypeapplication/pdf
dc.language.isoen_AU
dc.publisherCambridge University Press
dc.rights© 2018 The Authors and New Phytologist Trust
dc.sourceNew Phytologist
dc.titleCarbon allocation to major metabolites in illuminated leaves is not just proportional to photosynthesis when gaseous conditions (CO2 and O2) vary
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume218
dcterms.dateAccepted2017-11-29
dc.date.issued2018-01-18
local.identifier.absfor060705 - Plant Physiology
local.identifier.ariespublicationa383154xPUB9291
local.publisher.urlhttps://nph.onlinelibrary.wiley.com/
local.type.statusAccepted Version
local.contributor.affiliationAbadie, Cyril, College of Science, ANU
local.contributor.affiliationBathellier, Camille, College of Science, ANU
local.contributor.affiliationTcherkez, Guillaume, College of Science, ANU
dc.relationhttp://purl.org/au-research/grants/arc/FT140100645
local.bibliographicCitation.issue1
local.bibliographicCitation.startpage94
local.bibliographicCitation.lastpage106
local.identifier.doi10.1111/nph.14984
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2020-11-23T11:35:35Z
local.identifier.scopusID2-s2.0-85040747233
dcterms.accessRightsOpen Access
dc.provenancehttps://v2.sherpa.ac.uk/id/publication/15984..."Author accepted manuscript can be made open access on non-commercial institutional repository after 12 month embargo" from SHERPA/RoMEO site (as at 13.5.2021).
CollectionsANU Research Publications

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