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Constrained evolution of a bispecific enzyme: lessons for biocatalyst design

Sugrue, E.; Scott, C.; Jackson, C. J.


One of the central goals of protein design and engineering is to be able to accurately predict the effects of a mutation on stability and activity. However, the genetic context into which mutations are introduced can lead to complex interactions between the mutation and other amino acids and unpredictable, non-additive, effects. This phenomenon is known as intramolecular epistasis and has been shown to restrict evolutionary paths through laboratory directed evolution experiments and ancestral...[Show more]

CollectionsANU Research Publications
Date published: 2017
Type: Journal article
Source: Organic and Biomolecular Chemistry
DOI: 10.1039/C6OB02355J


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