Mustafa, SanamHannagan, JohnRigby, PaulPfleger, Kevin D. G.Corry, Ben2015-12-131083-3668http://hdl.handle.net/1885/77752Accurate quantification of Förster resonance energy transfer (FRET) using intensity-based methods is difficult due to the overlap of fluorophore excitation and emission spectra. Consequently, mechanisms are required to remove bleedthrough of the donor emAuthor/s retain copyrightKeywords: fluorescent dye; photoprotein; algorithm; article; cell strain HEK293; chemistry; fluorescence microscopy; fluorescence resonance energy transfer; genetics; human; light related phenomena; metabolism; methodology; statistics; Algorithms; Fluorescence ResoQuantitative Forster resonance energy transfer efficiency measurements using simultaneous spectral unmixing of excitation and emission spectra201310.1117/1.JBO.18.2.0260242016-02-24