McDonald, MeganWilliams , Angela HMilgate, AndrewPattemore, Julie A.Solomon, PeterHane , James K2015-12-131087-1845http://hdl.handle.net/1885/76723The wheat pathogen Zymoseptoria tritici possesses a large number of accessory chromosomes that may be present or absent in its genome. The genome of the reference isolate IPO323 has been assembled to a very high standard and contains 21 full length chromosome sequences, 8 of which represent accessory chromosomes. The IPO323 reference, when combined with low-cost next-generation sequencing and bioinformatics, can be used as a powerful tool to assess the presence or absence of accessory chromosomes. We present an outline of a range of bioinformatics techniques that can be applied to the analysis of presence-absence variation among accessory chromosomes across 13 novel isolates of Z. tritici.201510.1016/j.fgb.2015.04.0122015-12-11