Broersen, RobinStuart, Gregory2024-07-172024-07-172666-1667https://hdl.handle.net/1885/733713984In vivo whole-cell recording, when combined with morphological characterization after biocytin labeling, is a powerful technique to study subthreshold synaptic processing in cell-type-identified neuronal populations. Here, we describe steps for performing whole-cell recordings in the superior colliculus of urethane-anesthetized mice, a major visual processing region in the rodent brain. We detail two types of visual stimulation techniques: full-field light-emitting diode (LED) flashes and visual stimuli shown on monitors. While we focus on superior colliculus, this protocol is applicable to other brain areas.application/pdfen-AU© 2022 The authorshttp://creativecommons.org/licenses/ by-nc-nd/4.0/202310.1016/j.xpro.2022.1019632024-05-19Creative Commons Attribution licence