Cloning, expression, purification, crystallization and preliminary X-ray studies of a pyridoxine 5'-phosphate oxidase from Mycobacterium smegmatis

Jackson, Colin JTaylor, Matthew CTattersall, David BFrench, Nigel GCarr, Paul DOllis, DavidRussell, RobynOakeshott, John Graham2015-12-101744-3091http://hdl.handle.net/1885/51611Pyridoxine 5′-phosphate oxidases (PNPOxs) are known to catalyse the terminal step in pyridoxal 5′-phosphate biosynthesis in a flavin mononucleotide-dependent manner in humans and Escherichia coli. Recent reports of a putative PNPOx from MycobacteriumKeywords: pyridoxamine phosphate oxidase; selenomethionine; article; chemistry; crystallization; enzymology; Escherichia coli; genetics; human; isolation and purification; metabolism; methodology; molecular cloning; Mycobacterium smegmatis; X ray diffraction; Cloni FMN; Mycobacterium smegmatis; Pyridoxine 5'-phosphate oxidasesCloning, expression, purification, crystallization and preliminary X-ray studies of a pyridoxine 5'-phosphate oxidase from Mycobacterium smegmatis200810.1107/S17443091080115122015-12-09