Rockendorf, NielsBade, SteffenHirst, TimothyGorris, Hans H.Frey, Andreas2015-12-071043-1802http://hdl.handle.net/1885/27064A ganglioside GM1 probe bearing a dark-red fluorescent dye at the sphingosine moiety of the molecule was prepared by a convenient one-pot synthesis. The labeled GM1 permitted the detection of the natural ganglioside GM1 ligand Escherichia coli heat-labile enterotoxin subunit B (EtxB) in picomole quantities on a solid support. When an epitope mapping of several ganglioside binding proteins and protein fragments was performed by screening a cellulose membrane-bound synthetic library of 64 16mer peptides with the new probe, several peptides displaying ganglioside G M1 affinity could be identified. We consider the labeled glycolipid described herein a versatile tool for manifold biochemical investigations.Keywords: binding protein; cellulose; Escherichia coli enterotoxin; Escherichia coli heat labile enterotoxin subunit B; fluorescent dye; ganglioside GM1; ligand; peptide library; sphingosine; synthetic peptide; unclassified drug; amino acid sequence; article; bindi200710.1021/bc06023762015-12-07