Tan, Kar-ChunTrengove, RobertMaker, Garth L.Oliver, Richard PeterSolomon, Peter2015-12-071573-3882http://hdl.handle.net/1885/17746A recent comparative proteomics study identified the short-chain dehydrogenase (Sch1) as being required for asexual sporulation (Tan et al. Eukaryotic Cell 7:1916-1929, 2008). Metabolite profiling was undertaken on the mutant strains of Stagonospora nodorum lacking the Sch1 gene to help elucidate its role. Gas chromatography-mass spectrometry of the polar metabolites in the Sch1 mutants identified a secondary metabolite at a 200-fold greater concentration than observed in the wild-type strains. Comparative analysis of the secondary metabolite and the mycotoxin alternariol using ESI-MS/MS confirmed the identity of the compound as alternariol. This is the first report to confirm the presence of a mycotoxin in S. nodorum and compelling the field to consider the health implication of this disease.Keywords: Eukaryota; Phaeosphaeria nodorum Alternariol; Metabolomics; Mycotoxin; Pathogen; Stagonospora nodorum200910.1007/s11306-009-0158-22016-02-24