TEA, IllaMartineau, EstelleAntheaume, IngridLalande, JulieMauve, CarolineGilard, FrancoiseBarillé-Nion, SophieBlackburn, AnnekeTcherkez, Guillaume2018-08-292018-08-292045-2322http://hdl.handle.net/1885/146724Breast cancer is the most common cancer in women worldwide. Despite the information provided by anatomopathological assessment and molecular markers (such as receptor expression ER, PR, HER2), breast cancer therapies and prognostics depend on the metabolic properties of tumor cells. However, metabolomics have not provided a robust and congruent biomarker yet, likely because individual metabolite contents are insufficient to encapsulate all of the alterations in metabolic fluxes. Here, we took advantage of natural 13C and 15N isotope abundance to show there are isotopic differences between healthy and cancer biopsy tissues or between healthy and malignant cultured cell lines. Isotope mass balance further suggests that these differences are mostly related to lipid metabolism, anaplerosis and urea cycle, three pathways known to be impacted in malignant cells. Our results demonstrate that the isotope signature is a good descriptor of metabolism since it integrates modifications in C partitioning and N excretion altogether. Our present study is thus a starting point to possible clinical applications such as patient screening and biopsy characterization in every cancer that is associated with metabolic changes.The authors thank the support of the Agence Nationale de la Recherche through a research grant Jeunes Chercheurs (under contract 12-000101), the metabolomics platform CORSAIRE from the Biogenouest network, the funding from SATT (Nantes University Technology Transfer Services), from PLAISIR Pays de la Loire Association for International Structure on “Isotopomic Research” French Regional International Strategy Grant and the Australian Research Council through a Future Fellowship (under contract FT14 0100645).9 pagesapplication/pdf© 2016 The Author(s)http://creativecommons.org/licenses/by/4.0/breast cancer13C15Nisotopemetabolism2016-09-2810.1038/srep34251Creative Commons Attribution 4.0 International License