Maier, Alexander G.Schulreich, SebastianBremser, MartinaClayton, Christine2026-01-012026-01-010014-5793PubMed:11068037https://hdl.handle.net/1885/733801659Microbodies are single membrane-bound organelles found in eukaryotes from trypanosomes to man. Although they have diverse roles in metabolism, the mechanisms and molecules involved in membrane biogenesis and matrix protein import are conserved. Similarly, the basic mechanisms and structures involved in vesicular transport are similar throughout eukaryotic evolution. The PEX11 proteins are required for the division of microbodies in trypanosomes, yeast and mammals, and a role of coatomer in this process has been suggested. We show here that the binding of trypanosome, yeast and bovine coatomers to selected peptides is identical. Coatomer binds to the C-termini of trypanosome PEX11 and rat Pex11α, but not yeast Pex11p or human Pex11β. Mutations of the C-terminus of trypanosome PEX11 that eliminated coatomer binding did not affect function in yeast or trypanosomes. Thus binding of coatomer to the C-terminus of PEX11 is not required for PEX11 function. Copyright (C) 2000 Federation of European Biochemical Societies.This work was supported by the Deutsche Forschungsgemeinschaft (SFB352). The PCR mutagenesis was partly performed by Claudia Hartmann and a practical class (Hauptpraktikum B, ZMBH September 1998). We are indebted to Paul Michels (ICP, Brussels) for antisera, Cordula Harter (BZH) for antibodies against mammalian COP, Ralf Erdmann (antibody to yeast thiolase, plasmids and the pex11 Δ yeast strain) and Rainer Duden, University of Cambridge (antibody to yeast β′-COP). We are very grateful to Mark Carrington (University of Cambridge, UK) for providing the sequence of β′-COP prior to publication, Claudia Kruse for the yeast strain, Martina Ding for help in digital deconvolution microscopy, Luis Quijada and the members of Ralf Jansen’s laboratory (ZMBH) for their help in yeast genetics.5enCoatomerGlycosomeMicrobodyPEX11Trypanosome2000-11-0310.1016/S0014-5793(00)02132-30034602530