Busch, FlorianDeans, RossHolloway-Phillips, Meisha-MarikaFernie, Alisdair R.Bauwe, HermannWeber, Prof. Dr. Andreas P.M.2021-06-169781493972241http://hdl.handle.net/1885/237744Photorespiratory fluxes can be easily estimated by photosynthetic gas exchange using an infrared gas analyzer and applying the Farquhar, von Caemmerer, and Berry (Farquhar et al. Planta 149:78–90, 1980) photosynthesis model. For a more direct measurement of photorespiratory CO2 release from glycine decarboxylation, infrared gas analysis can be coupled to membrane-inlet mass spectrometry, capable of separating the total CO2 concentration into its 12CO2 and 13CO2 components in a continuous online fashion. This chapter discusses how to calculate rates of photorespiration from Rubisco kinetics and describes in detail a method for measuring the CO2 release from glycine decarboxylation using 13CO2.We would therefore like to thank the Deutsche Forschungsgemeinschaft and the 12 project partners and 10 associate partners for financially supporting the Promics project (DFG FOR1186) and thus for making this book possible.application/pdfen-AU© Springer Science+Business Media LLC 2017PhotorespirationPhotosynthetic gas exchangeMembrane-inlet mass spectrometryCarbon isotopesPhotosynthesis modelRubisco kinetics201710.1007/978-1-4939-7225-82020-11-23