Zafar, AnjumHardy, KristineWu, FanLi, JasmineRao, Sudha2026-01-012026-01-012213-5960ORCID:/0000-0003-1660-4477/work/182749144https://hdl.handle.net/1885/733798696The protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) induces transition of the epithelial MCF-7 cell line to a mesenchymal phenotype. A subset of the resulting mesenchymal cells has surface markers characteristics of a cancer stem cell (CSC) population. We profiled the transcriptome changes associated with the epithelial to mesenchymal transition and those that occurred in the CSC subset. Using a siRNA knockdown strategy, we examined the extent to which these changes were dependent on the PKC family member, PKC-θ. The importance of the cytoplasmic signaling role of this kinase is well established and in this study, we have shown by PKC-θ ChIP-sequencing analysis that this kinase has a dual role with the ability to also associate with chromatin on a subset of PKC-θ dependent genes. In the associated manuscript (Zafar et al., 2014 [5]) we presented evidence for the first time showing that this nuclear role of PKC-θ is also important for gene induction and mesenchymal/CSC phenotype. Here we describe the analysis associated with the transcriptome and ChIP-seq data presented in Zafar et al. (2014) [5] and uploaded to NCBI Gene Expression Omnibus (. GSE53335).We would like to thank Harpreet Vohra and Michael Devoy for the support with the FACS sorting and the financial support of the Endeavour Postgraduate Award (number 641_2008 ) and The Australian National University of Canberra PDF fellowship scheme.5enPublisher Copyright: © 2014 The Authors.ChIP-seqMicroarraysStem cell2015-03-0110.1016/j.gdata.2014.11.00284922938011