Loscha, KarinHerlt, AnthonyQi, RuhuOzawa, KiyoshiOtting, GottfriedHuber, Thomas2015-12-101433-7851http://hdl.handle.net/1885/60833A cell-free protein synthesis system from which the release factor RF1 has been selectively removed enables the facile incorporation of unnatural amino acids into proteins at difficult and multiple sites by optimized use of orthogonal tRNA/aminoacyl-tRNAKeywords: Cell free protein synthesis; Number of samples; Optimized use; protein modifications; Release factors; Resonance assignments; Synthetases; termination factor RF1; Trifluoromethyl; Amino acids; Nuclear magnetic resonance spectroscopy; Proteins; amino acid; cell-free protein synthesis; NMR spectroscopy; protein modifications; termination factor RF1; unnatural amino acids201210.1002/anie.2011082752016-02-24