Ridgway, MelanieShea, Kwong SumCihalova, DanielaMaier, Alexander2021-02-232021-02-232379-5042http://hdl.handle.net/1885/224414We developed a flow-cytometry-based method to separate and collect cocultured male and female Plasmodium falciparum gametocytes responsible for malaria transmission. The purity of the collected cells was estimated at >97% using flow cytometry, and sorted cells were observed by Giemsa-stained thin-smear and live-cell fluorescence microscopy. The expression of validated sex-specific markers corroborated the sorting strategy. Collected male and female gametocytes were used to confirm three novel sex-specific markers by quantitative real-time PCR that were more enriched in sorted male and female gametocyte populations than existing sex-specific markers. We also applied the method as a proof-of-principle drug screen that allows the identification of drugs that kill gametocytes in a sex-specific manner. Since the developed method allowed for the separation of male and female parasites from the same culture, we observed for the first time a difference in development time between the sexes: females developed faster than males. Hence, the ability to separate male and female gametocytes opens the door to a new field of sex-specific P. falciparum gametocyte biology to further our understanding of malaria transmission.Funding was provided by the Australian Research Council (DP180103212). M.C.R. is supported by the Australian Government Research Training Program Scholarship and The Australian National Universityapplication/pdfen-AU© 2020 Ridgway et al.https://creativecommons.org/licenses/by/4.0/Plasmodium falciparummalariagametocytestransmissiongenetic markersex202010.1128/mSphere.00671-202020-11-15Creative Commons Attribution 4.0 International license