Tanner, Nathan AHamdan, SamirJergic, SlobodanLoscha, KarinSchaeffer, PatrickDixon, Nicholasvan Oijen, Antoine M2015-12-101545-9993http://hdl.handle.net/1885/50659We present single-molecule studies of the Escherichia coli replication machinery. We visualize individual E. coli DNA polymerase III (Pol III) holoenzymes engaging in primer extension and leading-strand synthesis. When coupled to the replicative helicaseKeywords: DNA polymerase; DNA primase; DnaB helicase; holoenzyme; article; controlled study; DNA replication; Escherichia coli; nonhuman; priority journal; protein protein interaction; DNA Polymerase III; DNA Replication; DNA, Bacterial; DnaB Helicases; Endodeoxyri200810.1038/nsmb.13812015-12-09