Jo, Seung BinKim, Hyun KyungLee, Hae NimKim, Yu JinPatel, Kapil DevKnowles, Jonathan CampbellLee, Jung HwanSong, Minju2025-06-112025-06-11ORCID:/0000-0002-0393-9166/work/171153377http://www.scopus.com/inward/record.url?scp=85093907346&partnerID=8YFLogxKhttps://hdl.handle.net/1885/733759061Calcium silicate-based bioactive glass has received significant attention for use in various biomedical applications due to its excellent bioactivity and biocompatibility. However, the bioactivity of calcium silicate nanoparticle-incorporated bioactive dental sealer is not much explored. Herein, three commercially available bioactive root canal sealers (Endoseal MTA (EDS), Well-Root ST (WST), and Nishika Canal Sealer BG (NBG)) were compared with a resin-based control sealer (AH Plus (AHP)) in terms of physical, chemical, and biological properties. EDS and NBG showed 200 to 400 nm and 100 to 200 nm nanoparticle incorporation in the SEM image, respectively, and WST and NBG showed mineral deposition in Hank’s balanced salt solution after 28 days. The flowability and film thickness of all products met the ISO 3107 standard. Water contact angle, linear dimensional changes, and calcium and silicate ion release were significantly different among groups. All bioactive root canal sealers released calcium ions, while NBG released ~10 times more silicon ions than the other bioactive root canal sealers. Under the cytocompatible extraction range, NBG showed prominent cytocompatibility, osteogenecity, and angiogenecity compared to other sealers in vitro. These results indicate that calcium silicate nanoparticle incorporation in dental sealers could be a potential strategy for dental periapical tissue regeneration.Acknowledgments: This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Ministry of Science and ICT (2019R1C1C1002490 and NRF-2019R1C1C1003240), by the Global Research Development Center Program (2018K1A4A3A01064257), and by the Priority Research Center Program by the Ministry of Education (2019R1A6A1A11034536). The present research was also supported by a 2019 research fund from Dankook University for the University Innovation Support Program. Funding: This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Ministry of Science and ICT (2019R1C1C1002490 and NRF-2019R1C1C1003240), by the Global Research Development Center Program (2018K1A4A3A01064257), and by the Priority Research Center Program by the Ministry of Education (2019R1A6A1A11034536). The present research was also supported by a 2019 research fund from Dankook University for the University Innovation Support Program.19enPublisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.AngiogenicBioactiveOsteogenic inductionRoot canal sealersSilicate ions202010.3390/nano1009175085093907346