Dealing with intractable protein cores: protein sequencing of the Mcg IgG and the Yvo IgM heavy chain variable domains

Shaw, DenisShultz, BrandonRamsland, PaulEdmundson, Allen2015-12-132015-12-130952-3499http://hdl.handle.net/1885/94297The VH domains of two human monoclonal antibodies, designated Mcg IgGI(λ) and Yvo IgM(κ), were particularly intractable to standard protein sequencing protocols. Peptides liberated from the VH domains of these proteins, using standard enzymatic or chemiKeywords: dodecyl sulfate sodium; immunoglobulin G; immunoglobulin heavy chain; monoclonal antibody; Bence Jones protein; immunoglobulin; immunoglobulin M; myeloma immunoglobulins; paraprotein; amino acid sequence; polyacrylamide gel electrophoresis; protein degrad Antibody crystallography; Human monoclonal antibodies; Insoluble peptides; Protein sequencingDealing with intractable protein cores: protein sequencing of the Mcg IgG and the Yvo IgM heavy chain variable domains200210.1002/jmr.5962015-12-12