Cofactor promiscuity among F 420 -dependent reductases enables them to catalyse both oxidation and reduction of the same substrate

Lapalikar, Gauri VTaylor, Matthew CWarden, Andrew COnagi, HidekiHennessy, James EMulder, Roger JScott, ColinBrown, Susan ERussell, Robyn JEaston, ChristopherOakeshott , John2015-12-102044-4753http://hdl.handle.net/1885/61579The recently reported F420H2-dependent reductases (FDRs) catalyse the reduction of aflatoxins and coumarin via hydrogenation of the α,β-unsaturated moiety. We report that three FDRs (MSMEG-2027, MSMEG-6848 and MSMEG-3356) from Mycobacterium smegmatis alKeywords: Catalyse; Catalytic functions; Cofactors; Mycobacterium smegmatis; Oxidation and reduction; Structural isomers; Bacteria; Catalysis; Enzymes; Isomers; Oxidation; AflatoxinsCofactor promiscuity among F 420 -dependent reductases enables them to catalyse both oxidation and reduction of the same substrate201210.1039/c2cy20129a2016-02-24