Cui, JingPeuke, Andreas D.Tcherkez, GuillaumeLimami, Anis M.2022-11-020140-7791http://hdl.handle.net/1885/277972It has now been 61 years since phloem sap composition has first been described, using sap extracted from willow (Peel & Weatherley, 1959). In that study, using colorimetric methods, nitrate was found to be undetectable. Several subsequent studies also reported the absence of nitrate in other species such as castor bean (Ricinus communis) (Hall & Baker, 1972) and overviews of phloem sap composition established the generally very low, or undetectable, concentration in phloem sap nitrate (Ziegler, 1975).The authors acknowledge the financial support of the Région Pays dela Loire and Angers Loire Métropole via the grant Connect TalentIsoseed awarded to G.Tapplication/pdfen-AU© 2021 The authors202110.1111/pce.141162021-11-28