Selective 15 N-labeling of the side-chain amide groups of asparagine and glutamine for applications in paramagnetic NMR spectroscopy

Cao, ChanChen, Jia-LiangYang, YinHuang, FengSu, XunchengOtting, Gottfried2015-12-100925-2738http://hdl.handle.net/1885/63567The side-chain amide groups of asparagine and glutamine play important roles in stabilizing the structural fold of proteins, participating in hydrogen-bonding networks and protein interactions. Selective 15N-labeling of side-chain amides, however, can behttp://www.sherpa.ac.uk/romeo/issn/0925-2738/..."Author's post-print on any open access repository after 12 months after publication" from SHERPA/RoMEO site (as at 18/10/18). This is a post-peer-review, pre-copyedit version of an article published in [Journal of Biomolecular NMR]. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10858-014-9844-0Selective 15 N-labeling of the side-chain amide groups of asparagine and glutamine for applications in paramagnetic NMR spectroscopy201410.1007/s10858-014-9844-02015-12-10