Barros-Nepomuceno, Francisco WashingtonViana, Daniel de AraujoPinheiro, Daniel Pascoalinode Oliveira, Fatima de Cassia EvangelistaFerreira, Jamile Magalhaesde Queiroz, Maria GorettiMa, XingCavalcanti, Bruno CoelhoPessoa, ClaudiaBanwell, Martin2022-10-051860-7179http://hdl.handle.net/1885/274296The tambjamines are a small group of bipyrrolic alkaloids that, collectively, display a significant range of biological activities including antitumor, antimicrobial and immunosuppressive properties. The key objective of the present study was to undertake preclinical assessments of tambjamine J (T‐J) so as to determine its in vivo antitumor effects. To that end, sarcoma 180 cells were transplanted in mice and the impacts of the title compound then evaluated using a range of protocols including hematological, biochemical, histopathological, genotoxic and clastogenic assays. As a result it was established that this alkaloid has a significant therapeutic window and effectively reduces tumor growth (by 40 % and 79 % at doses of 10 and 20 mg/kg/day, respectively). In this regard it displays similar antitumor activity to the anticancer agent cyclophosphamide and alters animal weight in an analogous manner.The study was financed in part by the Coordenação de Aperfeiçoamento Pessoalde NívelSuperior– Brasil(CAPES)(Finance Code 001-Capes Print Process nr. 8887311918/2018-00).The authors are also grateful to Conselho Nacionalde Desenvolvi-mento Científico e Tecnológico– CNPq for financial support(Process nr. 440755/2018-2)as well as research sponsorship of CP(PQ-1B Process nr. 303102/2013-6).The Australian Research Council is thanked for variousgrantsand fellowships.application/pdfen-AU© 2020 Wiley-VCHGmbH202110.1002/cmdc.2020003872021-11-28