Emlyn-Jones, DanielWoodger, FionaPrice, Graeme (Dean)Whitney, Spencer2015-12-080032-0781http://hdl.handle.net/1885/30637In most cyanobacteria, the gene rbcX is co-transcribed with the rbcL and rbcS genes that code for the large and small subunits of ribulose-1,5- bisphosphate carboxylase/oxygenase (Rubisco). Previous co-expression studies in Escherichia coli of cyanobacterial Rubisco and RbcX have identified a chaperonin-like function for RbcX. The organization of the rbcLXS operon has, to a certain extent, precluded definitive gene function studies of rbcX in cyanobacteria. In Synechococcus PCC7942, however, rbcX is located >100 kb away from the rbcLS operon, providing an opportunity to examine the role of RbcX by insertional inactivation without interference from the Rubisco genes. Fully segregated Synechococcus PCC7942 ΔrbcXKeywords: chaperonin; ribulosebisphosphate carboxylase; vegetable protein; amino acid sequence; article; cell survival; cytology; Escherichia coli; gene; gene expression regulation; genetics; metabolism; molecular genetics; physiology; protein folding; Synechococcu Chaperone; Co-expression; Gene function; RbcX; Rubisco; Synechococcus200610.1093/pcp/pcl0282015-12-08