Berry, Richard P.Ibbotson, Michael R.2015-11-292015-11-291932-6203http://hdl.handle.net/1885/16895Three-dimensional digital atlases are rapidly becoming indispensible in modern biology. We used serial sectioning combined with manual registration and segmentation of images to develop a comprehensive and detailed three-dimensional atlas of the honeybee head-neck system. This interactive atlas includes skeletal structures of the head and prothorax, the neck musculature, and the nervous system. The scope and resolution of the model exceeds atlases previously developed on similar sized animals, and the interactive nature of the model provides a far more accessible means of interpreting and comprehending insect anatomy and neuroanatomy.This work was funded by the ARC Centre of Excellence in Vision Science (CE0561903) together with the Air Force Office of Scientific Research (FA 4869). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.© 2010 Berry, Ibbotson. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.animalsbeesextremitiesfemaleheadmovementneckneck musclesnervous systemmodels, anatomic2010-05-2410.1371/journal.pone.00107712015-12-09