Engineering of a bis-chelator motif into a protein α-helix for rigid lanthanide binding and paramagnetic NMR spectroscopy

Swarbrick, JamesUng, PhucSu, Xun-ChengMaleckis, AnsisChhabra, SandeepGraham, BimOtting, GottfriedHuber, Thomas2015-12-101359-7345http://hdl.handle.net/1885/58994Attachment of two nitrilotriacetic acid-based ligands to a protein α-helix in an i, i + 4 configuration produces an octadentate chelating motif that is able to bind paramagnetic lanthanide ions rigidly and with high affinity, leading to large pseudocontaKeywords: edetic acid; lanthanide; nitrilotriacetic acid; alpha helix; article; binding affinity; cross coupling reaction; ligand binding; nuclear magnetic resonance spectroscopy; protein binding; protein engineering; protein motif; protein protein interaction; AmiEngineering of a bis-chelator motif into a protein α-helix for rigid lanthanide binding and paramagnetic NMR spectroscopy201110.1039/c1cc11893e2016-02-24