Keniry, MaxOwen, Elisabeth2015-12-100014-5793http://hdl.handle.net/1885/63171Defining the molecular basis of the DNA sequence selectivity of polyamine binding is central to understanding polyamine-dependent gene expression. We have studied, by selective NMR experiments, the variation of spermine mobility and conformation in the presence of G-quadruplexes formed by sequences of the purine-rich strand of the c-Myc promoter, nuclease hypersensitivity element III1 (NHE III1). All the NHE quadruplexes restrict spermine mobility and induce a spermine conformational change but the most effective immobilisation occurs when all five G-tracts of the NHE III 1 are present. This suggests structure within the nucleotides flanking the G-quadruplex has a role in immobilising spermine.201410.1016/j.febslet.2014.04.0032015-12-10