Piggott, Maxine2021-05-061877-7252http://hdl.handle.net/1885/232513Macquarie perch (Macquaria australasica) are an endangered freshwater fish found in the Murray-Darling Basin, Australia. I developed a species-specific assay to detect Macquarie perch DNA in environmental samples. The assay is highly specific to detect Macquarie perch, and produced no amplification from 18 co-distributed species. Testing the assay on environmental water samples demonstrated positive detections where Macquarie perch are known to be present in low and moderate density.application/pdfen-AU© Springer Science+Business Media Dordrecht 2017eDNAEnvironmental DNAMacquarie perchMacquaria australasicaqPCR201710.1007/s12686-016-0666-02020-11-23