Periasamy, PravinTan, Jonathan K. HO'Neill, Helen2013-09-192013-09-190741-54001938-3673http://hdl.handle.net/1885/10501The main DC subsets in murine spleen arise from BM-derived precursors. Recently, a novel APC type was described in spleen. To determine if spleen contains the progenitors of this subset, a stromal coculture system was used to assess candidate progenitors for their hematopoietic potential. Here, the progenitor of that subset is identified as a spleen endogenous Lin_c-kit lo hematopoietic progenitor and is most highly enriched among the Lin_c-kit(lo)CD34+ subset. Dendritic-like cells produced in vitro functionally resemble the previously described in vivo equivalent subset with high endocytic capacity and capability for antigen-specific activation of CD8+ T cells but not CD4+ T cells.NHMRC (National Health and Medical Research Council of Australia)7 pagesThe Deputy Director, Office of Publications, Federation of American Societies for Experimental Biology (FASEB) does not give permission for this article to be deposited in an institutional repository - from email, dated 19/9/13hematopoiesisdendritic cellsstroma2012-10-2510.1189/jlb.05122602015-12-12