Earles, Jeffrey MasonBuckley, Thomas N.Brodersen, Craig R.Busch, FlorianCano, Francisco JavierChoat, BrendanEvans, John R.Farquhar, GrahamHarwood, RichardHuynh, MinhJohn, Grace P.Miller, Megan L.Rockwell, F. E.2019-06-241360-1385http://hdl.handle.net/1885/164176Leaves are a nexus for the exchange of water, carbon, and energy between terrestrial plants and the atmosphere. Research in recent decades has highlighted the critical importance of the underlying biophysical and anatomical determinants of CO2 and H2O transport, but a quantitative understanding of how detailed 3D leaf anatomy mediates within-leaf transport has been hindered by the lack of a consensus framework for analyzing or simulating transport and its spatial and temporal dynamics realistically, and by the difficulty of measuring within-leaf transport at the appropriate scales. We discuss how recent technological advancements now make a spatially explicit 3D leaf analysis possible, through new imaging and modeling tools that will allow us to address long-standing questions related to plant carbon–water exchange.This work was supported by the University of Sydney through the SREI 2020 scheme and by the ARC Centre for Translational Photosynthesis. M.M.B. and R.H. acknowledge the facilities of the Australian Microscopy and Microanalysis Research Facility at Sydney Microscopy and Microanalysis, and technical assistance from Timur Burykin and Elinor Goodman.application/pdfen-AU© 2018 Elsevier Ltdleaf anatomyleaf hydraulic conductancemesophyll conductancephotosynthesis3D201910.1016/j.tplants.2018.09.0052019-04-21