Ando, JunBautista, GodofredoSmith, NicholasFujita, KatsumasaDaria, Vincent Ricardo2015-12-042015-12-040034-6748http://hdl.handle.net/1885/17012We present optical trapping and surgery of living yeast cells using two operational modes of a single laser. We used a focused laser beam operating in continuous-wave mode for noninvasive optical trapping and manipulation of single yeast cell. We verified that such operational mode of the laser does not cause any destructive effect on yeast cell wall. By changing the operation of the laser to femtosecond-pulsed mode, we show that a tightly focused beam dissects the yeast cell walls via nonlinear absorption. Lastly, using the combined technique of optical microsurgery and trapping, we demonstrate intracellular organelle extraction and manipulation from a yeast cell. The technique established here will be useful as an efficient method for both surgery and manipulation of living cells using a single laser beam.The project has been funded by the Philippine Council for Advanced Science and Technology Research and Development PCASTRD. J. Ando acknowledges the support of the Japan Student Services Organization JASSO for the short-term student exchange promotion program.http://www.sherpa.ac.uk/romeo/issn/0034-6748..."Publishers version/PDF may be used on author's personal website, institutional website or institutional repository" from SHERPA/RoMEO site (as at 4/12/15). Copyright 2008 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Review of Scientific Instruments and may be found at https://doi.org/10.1063/1.2999542cell wallcytological techniquesmicroscopy, videooptics and photonicsorganellessaccharomyces cerevisiaelasers2008-10-2010.1063/1.29995422015-12-08