Marchetti, Rosa V.Lehane, Adele M.Shafik, Sarah H.Winterberg, MarkusMartin, Rowena E.Kirk, Kiaran2015-07-132015-07-132041-1723http://hdl.handle.net/1885/14283The intraerythrocytic malaria parasite relies primarily on glycolysis to fuel its rapid growth and reproduction. The major byproduct of this metabolism, lactic acid, is extruded into the external medium. In this study, we show that the human malaria parasite Plasmodium falciparum expresses at its surface a member of the microbial formate-nitrite transporter family (PfFNT), which, when expressed in Xenopus laevis oocytes, transports both formate and lactate. The transport characteristics of PfFNT in oocytes (pH-dependence, inhibitor-sensitivity and kinetics) are similar to those of the transport of lactate and formate across the plasma membrane of mature asexual-stage P. falciparum trophozoites, consistent with PfFNT playing a major role in the efflux of lactate and hence in the energy metabolism of the intraerythrocytic parasite.This work was supported by grants from the Australian National Health and Medical Research Council (NHMRC; 316933 and 525428 to K.K. and 1007035 to R.E.M.), and by the L’Ore´al Australia For Women in Science programme (R.E.M.). A.M.L. was supported by an NHMRC Overseas Biomedical Fellowship (585519) and R.E.M. was supported by NHMRC Australian Biomedical Fellowships (520320 and 1053082).7 pages© 2015 Macmillan Publishers Limited. http://www.sherpa.ac.uk/romeo/issn/2041-1723/..."Publisher's version/PDF may be used. On author's personal website, institutional repository or open access repository" from SHERPA/RoMEO site (as at 13/07/15)A lactate and formate transporter in the intraerythrocytic malaria parasite, Plasmodium falciparum2015-03-3110.1038/ncomms77212015-12-08