Nitsche, ChristophOtting, Gottfried2020-09-012020-09-010959-440Xhttp://hdl.handle.net/1885/209180NMR spectroscopy is an established tool in drug discovery, but its strength is commonly regarded to be largely confined to the early stages of hit discovery and fragment based drug design, where NMR offers unique capabilities of characterizing the binding modes of ligand molecules that bind sufficiently weakly to be in rapid exchange between bound and free state. Here we, first, provide a meta-review of recent reviews on NMR studies of ligand binding and, second, review recent progress towards NMR characterization of the ligand binding mode in stable protein-ligand complexes, with particular emphasis on the global positioning system (GPS) approach enabled by paramagnetic lanthanide tags.CN thanks the Alexander von Humboldt Foundation for a Feodor LynenFellowship and GO thanks the Australian Research Council for a LaureateFellowship. Financial project support by the Australian Research Council isgratefully acknowledged.application/pdfen-AU© 2017 Elsevier Ltdhttps://creativecommons.org/licenses/by-nc-nd/4.0/animalsbinding sitesdrug designdrug discoverydrugs, investigationalhumanslanthanoid series elementsligandsmagnetic resonance spectroscopymodels, molecularprotein bindingprotein conformation, alpha-helicalprotein conformation, beta-strandprotein interaction domains and motifsproteinsstaining and labeling201810.1016/j.sbi.2017.09.001CC BY-NC-ND