Weintraub, Bennet C.Jun, JesseBishop, AdrianShokat, KevanThomas, MGoodnow, Christopher2015-12-130022-1007http://hdl.handle.net/1885/89904Signal transduction through the B cell antigen receptor (BCR) is altered in B cells that express a receptor that recognizes self-antigen. To understand the molecular basis for the change in signaling in autoreactive B cells, a transgenic model was used to isolate a homogeneous population of tolerant B lymphocytes. These cells were compared with a similar population of naive B lymphocytes. We show that the BCR from naive B cells enters a detergent-insoluble domain of the cell within 6 s after antigen binding, before a detectable increase in BCR phosphorylation. This fraction appeals to be important for signaling because it is enriched for lyn kinase but lacks CD45 tyrosine phosphatase and because the BCR that moves into this domain becomes more highly phosphorylated. Partitioning of the BCR into this fraction is unaffected by src family kinase inhibition. Tolerant B cells do not efficiently partition the BCR into the detergent-insoluble domain, providing an explanation for their reduced tyrosine kinase activation and calcium flux in response to antigen. These results identify an early, regulated step in antigen receptor signaling and self-tolerance.Keywords: autoantigen; B lymphocyte receptor; calcium; protein tyrosine kinase; animal cell; antigen recognition; article; B lymphocyte; calcium transport; controlled study; enzyme activation; gene expression; lymphocyte subpopulation; mouse; nonhuman; priority jou Anergy; B cell antigen receptor signaling; Membrane raft; Self-tolerance; Tyrosine phosphorylation200010.1084/jem.191.8.14432015-12-12