Alsharifi, MohammedLobigs, MarioBettadapura, JayaramKoskinen, AulikkiMullbacher, Arno2015-12-080022-1317http://hdl.handle.net/1885/30513Previously, we have shown that mice defective in granule exocytosis and/ or Fas.L/Fas-mediated cytolytic pathways are significantly more resistant to alphavirus, Semliki Forest virus (SFV), infection compared with wild-type mice. Here, we evaluated SFV replication in different tissues of mice defective in both cytolytic pathways (perf-/-xg/d) relative to that in wild-type counterparts and found that viral replication in perf-/-xg/d mice is remarkably restricted. Although the mechanism responsible for this observation is yet to be established, the lower virus titres found in these mice indicate that the role of cytolytic effector molecules in antiviral immunity needs to be re-evaluated.Keywords: Fas ligand; perforin; animal experiment; animal model; animal tissue; article; brain tissue; controlled study; cytolysis; mouse; muscle; nonhuman; priority journal; Semliki Forest alphavirus; signal transduction; spleen; tissue specificity; Togavirus infe200810.1099/vir.0.83626-02015-12-08