Saptari, R. T.Susila, H.2026-07-032026-07-031054-5476ORCID:/0000-0002-4621-064X/work/219175986https://hdl.handle.net/1885/733812440Although the application of exogenous hormones and the status of endogenous hormones play a crucial factor in somatic embryogenesis (SE), our understanding of the status of endogenous hormones and the molecular framework for hormone biosynthesis during SE are still elusive. With a data mining approach, we analyzed public RNA-seq data of SE from two model plants, Arabidopsis thaliana and Oryza sativa var. Japonica, which showed that several plant hormone biosynthesis genes had distinct patterns during SE. The expressions of auxin, cytokinin, gibberellin, and ethylene biosynthesis genes were induced during SE in A. thaliana, while only jasmonic acid showed an expression increase during somatic embryogenesis in both plants. Furthermore, by analyzing public RNA sequencing and DNA affinity purification sequencing (DAP-seq), we revealed some possibilities of the role of ethylene during SE through the activity of EIN3, by increasing the expression level of major transcription factors of somatic embryo development such as AGL15, BBM, and FUS3.We are grateful to Z. Nasim (Korea University, South Korea) for his technical advices on bioinformatics analysis and to Dr. R. A. Putranto (Indonesian Research Institute for Biotechnology and Bioindustry, Indonesia) for the discussion during manuscript preparation. Funding information This paper is also made possible through the support of Indonesian Endowment Fund for Education (LPDP) to R. T. Saptari.14enPublisher Copyright: © 2018, The Society for In Vitro Biology.Data miningPhytohormonesSomatic embryogenesisTranscriptomics2019-04-1510.1007/s11627-018-9947-585055984328