Tang, T. Y.DoraCecchi, DarioFracasso, GiorgioAccardi, DavideCoutable-Pennarun, AngeliqueMansy, Sheref S.Perriman, Adam W.Anderson, J. L.RossMann, Stephen2026-03-272026-03-27PubMed:29091420ORCID:/0000-0003-2205-9364/work/209602524https://hdl.handle.net/1885/733807951A gene-directed chemical communication pathway between synthetic protocell signaling transmitters (lipid vesicles) and receivers (proteinosomes) was designed, built and tested using a bottom-up modular approach comprising small molecule transcriptional control, cell-free gene expression, porin-directed efflux, substrate signaling, and enzyme cascade-mediated processing.We thank the Light Microscopy Facility at MPI-CBG, BBSRC/EPSRC-funded BrisSynBio Research Centre (BB/L01386X/1) for the use of well plate readers. The authors were supported by grants from the BrisSynBio (a BBSRC/EPSRC synthetic Biology research centre) (T.Y.D.T., A.C., A.W.P., J.L.R.A., S.M.); the MaxSynBio consortium (T.Y.D.T.) (jointly funded by the Federal Ministry of Education and Research of Germany and the Max Planck Society) and MPG (D.A.); Erasmus (G.F.); Armenise-Harvard Foundation and the Simons Foundation (290358) (S.S.M. and D.C.) and a EPSRC Early Career Fellowship EP/K026720/1 (A.W.P.).8enPublisher Copyright: © 2017 American Chemical Society.cell-free expressioncommunicationenzyme cascadegenetic switcheslipid vesiclesproteinosomesα-hemolysin2017-11-0110.1021/acssynbio.7b0030685042200167