Wu, PeterOzawa, KiyoshiLim, Siew PVasudevan, Subhash G.Dixon, Nicholas EdwardOtting, Gottfried2015-12-071433-7851http://hdl.handle.net/1885/28139Quick and clean: Excellent protein yields in cell-free protein synthesis can be obtained by using polymerase chain reaction (PCR) amplified DNA templates, provided that the templates are designed for cyclization. This eliminates time-consuming cloning steps and allows one to go from complementary DNA to the protein NMR spectrum in 24 h. (Graph Presented).Keywords: Cell culture; Cyclization; Enzyme kinetics; Proteins; Reaction kinetics; Transcription; Cell-free protein synthesis; High-throughput methods; Polymerase chain reaction; DNA; cell extract; DNA; article; cell free system; cytology; Escherichia coli; genetic Cell-free protein synthesis; High-throughput methods; NMR spectroscopy; Polymerase chain reaction200710.1002/anie.2006052372015-12-07