Roberts, SeanWilliams, ClareRoy, SreejaFuruya, Yoichi2023-09-052023-09-052331-8325http://hdl.handle.net/1885/298232Asthma is a global problem that affects millions of individuals. An increased risk of respiratory viral and bacterial infections is one of the complications of asthma. We recently reported that mice with ovalbumin-induced allergic airway disease (AAD) are protected against influenza-Streptococcus pneumoniae co-infection. Here, we describe in detail a protocol on how to induce AAD and influenza-S. pneumoniae co-infection in mice and to evaluate the specific roles of asthma on immunity to viral and bacterial pathogens in the hope of translating findings to benefit asthmatic individuals.An abbreviated protocol was previously published in Roberts et al. (2019). Y. F. was supported by the American Lung Association Biomedical Research Grant (RG341974) and is supported by American Heart Association Scientist Development Grant (17SDG33630188) and NIH (1R56AI146434-01).application/pdfen-AU© 2020 The authorsAsthmaAllergic airway diseaseInfluenzaStreptococcus pneumoniaeCo-infection202010.21769/BioProtoc.35832022-07-24