Dale, C JaneThomson, ScottDe Rose, RobertMedveczky, CPamungkas, JokoBoyle, David BRamshaw, IanKent, Stephen JRanasinghe, Charani2015-12-131597451681http://hdl.handle.net/1885/85195Induction of HIV-specific T-cell responses by vaccines may facilitate efficient control of HIV replication. Plasmid DNA vaccines and recombinant fowlpox virus (rFPV) vaccines are promising HIV-1 vaccine candidates, although delivering either vaccine alone may be insufficient to induce sufficient T-cell responses. A consecutive immunization strategy, known as "prime-boost," involving priming with DNA and boosting with rFPV vaccines encoding multiple common HIV antigens, is used to induce broad and high-level T-cell immunity and ameliorate AIDS in macaques. This vaccine strategy is proceeding to clinical trials. This chapter describes the use of prime-boost vaccines to induce T-cell responses against HIV-1 and protective immunity against AIDS in macaques. Methods for the construction of the vaccines, the use of animal models, and the detection of immune responses are described.Keywords: DNA vaccine; Human immunodeficiency virus antigen; Human immunodeficiency virus vaccine; acquired immune deficiency syndrome; animal; Bagg albino mouse; cellular immunity; disease model; dose response; drug effect; Fowlpox virus; genetics; human; Human im20062015-12-12