Kucharski, RobertBall, EldonHayward, DavidMaleszka, Ryszard2015-12-130378-1119http://hdl.handle.net/1885/89275We have cloned and characterized a cDNA encoding a putative glutamate transporter, Am-EAAT, from the brain of the honeybee, Apis mellifera. The 543-amino-acid AmEAAT gene product shares the highest sequence identity (54%) with the human EAAT2 subtype. Am-EAAT is expressed predominantly in the brain, and its transcripts are abundant in the optic lobes and inner compact Kenyon cells of the mushroom bodies (MBs), with most other regions of the brain showing lower levels of Am-EAAT expression. High levels of Am-EAAT message are found in pupal stages, possibly indicating a role for glutamate in the developing brain. (C) 2000 Elsevier Science B.V. All rights reserved.Keywords: complementary DNA; excitatory amino acid; glutamate transporter; animal tissue; article; brain region; controlled study; DNA sequence; electrophoresis; gene expression; honeybee; immunoreactivity; in situ hybridization; molecular cloning; nonhuman; Northe Apis mellifera; EAAT; Kenyon cells; Mushroom bodies200010.1016/S0378-1119(99)00503-X2015-12-12